Hotspot

rps4-trnL(UUA)

LSC · 46158-47211 · 1054 bp

Back to Echinacanthus

Pi 0.0411

MarkerSeek score 80.00

Primer available yes

Label rps4-trnL(UUA)

Feature Metadata

label_name: rps4-trnL(UUA)
feature_type: igs
region: LSC
length_bp: 1054
variable_sites: 58
pi: 0.0411
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL(UUA)_p1	ATCGCTTCCAGCTTTAGTCC	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.7	58.2	1178-1303 bp	1.0	83.2
rps4-trnL(UUA)_p2	AGCGCGATTGGTTTCTAAGA	CCGTAGCGTCTACCGATTTC	45.0	55.0	57.6	58.2	1197-1322 bp	1.0	82.6
rps4-trnL(UUA)_p3	GTTAGTCCTGGTAAAGCCCC	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.6	58.2	1150-1275 bp	1.0	82.5
rps4-trnL(UUA)_p4	ATCGCTTCCAGCTTTAGTCC	CGTCTACCGATTTCGCCATA	50.0	50.0	57.7	57.6	1172-1297 bp	1.0	81.9
rps4-trnL(UUA)_p5	ATCGCTTCCAGCTTTAGTCC	ATTCCGGGGTTTCTCTGGAT	50.0	50.0	57.7	58.4	1250-1375 bp	1.0	81.7