Hotspot

ycf3-trnS(GGA)

LSC · 44534-45132 · 599 bp

Back to Echinacanthus

Pi 0.0544

MarkerSeek score 81.00

Primer available yes

Label ycf3-trnS(GGA)

Feature Metadata

label_name: ycf3-trnS(GGA)
feature_type: igs
region: LSC
length_bp: 599
variable_sites: 48
pi: 0.0544
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS(GGA)_p1	TCTCCTGAGGTTGTCGGAAT	CTACGCCTTGAACCACTCG	50.0	57.9	58.1	58.3	724-745 bp	1.0	75.9
ycf3-trnS(GGA)_p2	TTCTCCTGAGGTTGTCGGAA	CTACGCCTTGAACCACTCG	50.0	57.9	58.3	58.3	725-746 bp	1.0	74.1
ycf3-trnS(GGA)_p3	TTTCTCCTGAGGTTGTCGGA	CTACGCCTTGAACCACTCG	50.0	57.9	58.3	58.3	726-747 bp	1.0	74.1
ycf3-trnS(GGA)_p4	TCTCCTGAGGTTGTCGGAAT	CTTGAACCACTCGGCCATC	50.0	57.9	58.1	58.5	718-739 bp	1.0	73.9
ycf3-trnS(GGA)_p5	TCTCCTGAGGTTGTCGGAAT	GCTACGCCTTGAACCACTC	50.0	57.9	58.1	58.5	725-746 bp	1.0	73.7