Hotspot

petN-psbM

LSC · 29032-30027 · 996 bp

Back to Echinacanthus

Pi 0.0469

MarkerSeek score 82.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 996
variable_sites: 66
pi: 0.0469
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	TCATTCTAGTTCCTACCGCCT	50.0	47.6	58.0	58.0	1092-1110 bp	1.0	79.4
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	TCATTCTAGTTCCTACCGCCT	50.0	47.6	58.0	58.0	1090-1108 bp	1.0	79.4
petN-psbM_p3	ACTCGTAGTGTGGGGAAGAA	TCTAGTTCCTACCGCCTTTCT	50.0	47.6	58.0	57.9	1088-1106 bp	1.0	78.9
petN-psbM_p4	TCGTAGTGTGGGGAAGAAGT	TCTAGTTCCTACCGCCTTTCT	50.0	47.6	58.0	57.9	1086-1104 bp	1.0	78.9
petN-psbM_p5	CGTAGTGTGGGGAAGAAGTG	TCATTCTAGTTCCTACCGCCT	55.0	47.6	57.9	58.0	1089-1107 bp	1.0	78.8