Hotspot

rpoB-trnC(GCA)

LSC · 26779-27981 · 1203 bp

Back to Echinacanthus

Pi 0.0503

MarkerSeek score 80.60

Primer available yes

Label rpoB-trnC(GCA)

Feature Metadata

label_name: rpoB-trnC(GCA)
feature_type: igs
region: LSC
length_bp: 1203
variable_sites: 85
pi: 0.0503
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC(GCA)_p1	CGAGCCCATATCCTTGCTTT	CCGAGGAAGAATTCTGCGAA	50.0	50.0	58.0	58.0	1812-1857 bp	1.0	87.1
rpoB-trnC(GCA)_p2	TGCCAAACCCCAATCAATGA	CGCTTCTACCGTGGATTCAA	45.0	50.0	58.0	58.0	1584 bp	0.333333	53.1
rpoB-trnC(GCA)_p3	TGCCAAACCCCAATCAATGA	CCGAGGAAGAATTCTGCGAA	45.0	50.0	58.0	58.0	1441 bp	0.333333	53.1
rpoB-trnC(GCA)_p4	CGAGCCCATATCCTTGCTTT	CGCTTCTACCGTGGATTCAA	50.0	50.0	58.0	58.0	2000 bp	0.333333	53.1
rpoB-trnC(GCA)_p5	TGCCAAACCCCAATCAATGA	ACAAAGCATCAACGACCTGT	45.0	45.0	58.0	58.0	1485 bp	0.333333	52.9