primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score rps16-trnQ(UUG)_p1 rps16-trnQ(UUG) 1 TGCTTTCTACCACATCGTTTCA GAGGTTCGAATCCTTCCGTC 22 20 40.9 55.0 58.3 58.1 0.0 16.9 2.341638 5753 6848 1129-1172 1.000000 71.8 rps16-trnQ(UUG)_p2 rps16-trnQ(UUG) 2 GCTTTCTACCACATCGTTTCA GAGGTTCGAATCCTTCCGTC 21 20 42.9 55.0 56.6 58.1 0.0 16.9 2.516846 5753 6848 1128-1171 1.000000 70.4 rps16-trnQ(UUG)_p3 rps16-trnQ(UUG) 3 TGCTTTCTACCACATCGTTTC GAGGTTCGAATCCTTCCGTC 21 20 42.9 55.0 56.6 58.1 0.0 16.9 2.516846 5753 6848 1129-1172 1.000000 70.4 rps16-trnQ(UUG)_p4 rps16-trnQ(UUG) 4 TGCTTTCTACCACATCGTTT GAGGTTCGAATCCTTCCGTC 20 20 40.0 55.0 55.3 58.1 0.0 16.9 2.778917 5753 6848 1129-1172 1.000000 68.3 rps16-trnQ(UUG)_p5 rps16-trnQ(UUG) 5 GCTTTCTACCACATCGTTTCAA GAGGTTCGAATCCTTCCGTC 22 20 40.9 55.0 57.3 58.1 0.0 16.9 2.818539 5753 6848 1128-1171 1.000000 68.0 rpoB-trnC(GCA)_p1 rpoB-trnC(GCA) 1 CGAGCCCATATCCTTGCTTT CCGAGGAAGAATTCTGCGAA 20 20 50.0 50.0 58.0 58.0 0.0 9.2 0.037273 26779 27981 1812-1857 1.000000 87.1 rpoB-trnC(GCA)_p2 rpoB-trnC(GCA) 2 TGCCAAACCCCAATCAATGA CGCTTCTACCGTGGATTCAA 20 20 45.0 50.0 58.0 58.0 0.0 0.0 0.025844 26779 27981 1584-1584 0.333333 53.1 rpoB-trnC(GCA)_p3 rpoB-trnC(GCA) 3 TGCCAAACCCCAATCAATGA CCGAGGAAGAATTCTGCGAA 20 20 45.0 50.0 58.0 58.0 0.0 9.2 0.031407 26779 27981 1441-1441 0.333333 53.1 rpoB-trnC(GCA)_p4 rpoB-trnC(GCA) 4 CGAGCCCATATCCTTGCTTT CGCTTCTACCGTGGATTCAA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.031710 26779 27981 2000-2000 0.333333 53.1 rpoB-trnC(GCA)_p5 rpoB-trnC(GCA) 5 TGCCAAACCCCAATCAATGA ACAAAGCATCAACGACCTGT 20 20 45.0 45.0 58.0 58.0 0.0 0.0 0.053943 26779 27981 1485-1485 0.333333 52.9 trnC(GCA)-petN_p1 trnC(GCA)-petN 1 TTTCCCCAGTTCAAATCCGG CAGCCCAAGCGAGATTTACT 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.111966 28053 28941 946-959 1.000000 89.1 trnC(GCA)-petN_p2 trnC(GCA)-petN 2 TTTCCCCAGTTCAAATCCGG ACTTCTTCCCCACACTACGA 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.087498 28053 28941 999-1012 1.000000 88.9 trnC(GCA)-petN_p3 trnC(GCA)-petN 3 TTTCCCCAGTTCAAATCCGG TTCTTCCCCACACTACGAGT 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.087498 28053 28941 997-1010 1.000000 88.9 trnC(GCA)-petN_p4 trnC(GCA)-petN 4 TTTCCCCAGTTCAAATCCGG AGGTCCGGTTTTCCTCACTA 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.101935 28053 28941 1329-1352 0.666667 73.9 trnC(GCA)-petN_p5 trnC(GCA)-petN 5 TTTCCCCAGTTCAAATCCGG AAAGGTCCGGTTTTCCTCAC 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.105072 28053 28941 1331-1354 0.666667 73.9 petN-psbM_p1 petN-psbM 1 ACTCGTAGTGTGGGGAAGAA TCATTCTAGTTCCTACCGCCT 20 21 50.0 47.6 58.0 58.0 0.0 0.0 1.033465 29032 30027 1092-1110 1.000000 79.4 petN-psbM_p2 petN-psbM 2 TCGTAGTGTGGGGAAGAAGT TCATTCTAGTTCCTACCGCCT 20 21 50.0 47.6 58.0 58.0 0.0 0.0 1.033465 29032 30027 1090-1108 1.000000 79.4 petN-psbM_p3 petN-psbM 3 ACTCGTAGTGTGGGGAAGAA TCTAGTTCCTACCGCCTTTCT 20 21 50.0 47.6 58.0 57.9 0.0 0.0 1.102712 29032 30027 1088-1106 1.000000 78.9 petN-psbM_p4 petN-psbM 4 TCGTAGTGTGGGGAAGAAGT TCTAGTTCCTACCGCCTTTCT 20 21 50.0 47.6 58.0 57.9 0.0 0.0 1.102712 29032 30027 1086-1104 1.000000 78.9 petN-psbM_p5 petN-psbM 5 CGTAGTGTGGGGAAGAAGTG TCATTCTAGTTCCTACCGCCT 20 21 55.0 47.6 57.9 58.0 0.0 0.0 1.108380 29032 30027 1089-1107 1.000000 78.8 trnD(GUC)-trnY(GUA)_p1 trnD(GUC)-trnY(GUA) 1 CGGTGCTCTGACCAATTGAA TGGCAATATGTCTACGCTGG 20 20 50.0 50.0 58.5 57.8 7.1 0.0 0.716274 30711 30790 150-158 1.000000 79.4 trnD(GUC)-trnY(GUA)_p2 trnD(GUC)-trnY(GUA) 2 CGGTGCTCTGACCAATTGAA GGCAATATGTCTACGCTGGT 20 20 50.0 50.0 58.5 57.8 7.1 0.0 0.717825 30711 30790 149-157 1.000000 79.4 trnD(GUC)-trnY(GUA)_p3 trnD(GUC)-trnY(GUA) 3 CGGTGCTCTGACCAATTGAA GGGGACGGACTGTAAATTCG 20 20 50.0 55.0 58.5 58.4 7.1 0.0 0.828828 30711 30790 171-179 1.000000 77.9 trnD(GUC)-trnY(GUA)_p4 trnD(GUC)-trnY(GUA) 4 CGGTGCTCTGACCAATTGAA GGGACGGACTGTAAATTCGT 20 20 50.0 50.0 58.5 57.6 7.1 0.0 0.852505 30711 30790 170-178 1.000000 77.7 trnD(GUC)-trnY(GUA)_p5 trnD(GUC)-trnY(GUA) 5 CGGTGCTCTGACCAATTGAA CGGACTGTAAATTCGTTGGC 20 20 50.0 50.0 58.5 57.5 7.1 0.0 0.998018 30711 30790 166-174 1.000000 76.7 trnE(UUC)-trnT(GGU)_p1 trnE(UUC)-trnT(GGU) 1 CGTTGCCTCCTTGAAAGAGA GAACCGATGACTTACGCCTT 20 20 50.0 50.0 57.8 58.0 0.0 0.0 0.175803 31007 31621 719-741 1.000000 85.0 trnE(UUC)-trnT(GGU)_p2 trnE(UUC)-trnT(GGU) 2 CGTTGCCTCCTTGAAAGAGA CCATGGCGTTACTCTACCAC 20 20 50.0 55.0 57.8 58.1 0.0 0.0 0.233900 31007 31621 698-720 1.000000 84.8 trnE(UUC)-trnT(GGU)_p3 trnE(UUC)-trnT(GGU) 3 CGTTGCCTCCTTGAAAGAGA TGGCGTTACTCTACCACTGA 20 20 50.0 50.0 57.8 58.1 0.0 0.0 0.256054 31007 31621 695-717 1.000000 84.6 trnE(UUC)-trnT(GGU)_p4 trnE(UUC)-trnT(GGU) 4 CGTTGCCTCCTTGAAAGAGA CCGATGACTTACGCCTTACC 20 20 50.0 55.0 57.8 58.2 0.0 0.0 0.320736 31007 31621 716-738 1.000000 83.9 trnE(UUC)-trnT(GGU)_p5 trnE(UUC)-trnT(GGU) 5 CGTTGCCTCCTTGAAAGAGA TTGAACCGATGACTTACGCC 20 20 50.0 50.0 57.8 58.3 0.0 0.0 0.452638 31007 31621 721-743 1.000000 82.8 trnG(GCC)-trnfM(CAU)_p1 trnG(GCC)-trnfM(CAU) 1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 20 20 50.0 50.0 58.1 58.0 0.0 4.2 0.096882 36631 36798 231-278 1.000000 88.1 trnG(GCC)-trnfM(CAU)_p2 trnG(GCC)-trnfM(CAU) 2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 20 20 50.0 50.0 58.1 58.0 0.0 4.2 0.096882 36631 36798 232-279 1.000000 88.1 trnG(GCC)-trnfM(CAU)_p3 trnG(GCC)-trnfM(CAU) 3 AGAAGATGCGGGTTCGATTC GTCACGGGTTCAAATCCTGT 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.166466 36631 36798 225-272 1.000000 87.6 trnG(GCC)-trnfM(CAU)_p4 trnG(GCC)-trnfM(CAU) 4 AGAAGATGCGGGTTCGATTC GGGTAGAGCAGTTTGGTAGC 20 20 50.0 55.0 58.1 58.0 0.0 0.0 0.084264 36631 36798 267-314 1.000000 87.2 trnG(GCC)-trnfM(CAU)_p5 trnG(GCC)-trnfM(CAU) 5 GAGAAGATGCGGGTTCGATT GGGTAGAGCAGTTTGGTAGC 20 20 50.0 55.0 58.1 58.0 0.0 0.0 0.084264 36631 36798 268-315 1.000000 87.2 ycf3-trnS(GGA)_p1 ycf3-trnS(GGA) 1 TCTCCTGAGGTTGTCGGAAT CTACGCCTTGAACCACTCG 20 19 50.0 57.9 58.1 58.3 0.0 0.0 1.323268 44534 45132 724-745 1.000000 75.9 ycf3-trnS(GGA)_p2 ycf3-trnS(GGA) 2 TTCTCCTGAGGTTGTCGGAA CTACGCCTTGAACCACTCG 20 19 50.0 57.9 58.3 58.3 0.0 0.0 1.549797 44534 45132 725-746 1.000000 74.1 ycf3-trnS(GGA)_p3 ycf3-trnS(GGA) 3 TTTCTCCTGAGGTTGTCGGA CTACGCCTTGAACCACTCG 20 19 50.0 57.9 58.3 58.3 0.0 0.0 1.549797 44534 45132 726-747 1.000000 74.1 ycf3-trnS(GGA)_p4 ycf3-trnS(GGA) 4 TCTCCTGAGGTTGTCGGAAT CTTGAACCACTCGGCCATC 20 19 50.0 57.9 58.1 58.5 0.0 2.2 1.590161 44534 45132 718-739 1.000000 73.9 ycf3-trnS(GGA)_p5 ycf3-trnS(GGA) 5 TCTCCTGAGGTTGTCGGAAT GCTACGCCTTGAACCACTC 20 19 50.0 57.9 58.1 58.5 0.0 0.0 1.604335 44534 45132 725-746 1.000000 73.7 rps4-trnL(UUA)_p1 rps4-trnL(UUA) 1 ATCGCTTCCAGCTTTAGTCC CCGTAGCGTCTACCGATTTC 20 20 50.0 55.0 57.7 58.2 0.0 10.2 0.565978 46158 47211 1178-1303 1.000000 83.2 rps4-trnL(UUA)_p2 rps4-trnL(UUA) 2 AGCGCGATTGGTTTCTAAGA CCGTAGCGTCTACCGATTTC 20 20 45.0 55.0 57.6 58.2 12.1 10.2 0.628532 46158 47211 1197-1322 1.000000 82.6 rps4-trnL(UUA)_p3 rps4-trnL(UUA) 3 GTTAGTCCTGGTAAAGCCCC CCGTAGCGTCTACCGATTTC 20 20 55.0 55.0 57.6 58.2 0.0 10.2 0.663442 46158 47211 1150-1275 1.000000 82.5 rps4-trnL(UUA)_p4 rps4-trnL(UUA) 4 ATCGCTTCCAGCTTTAGTCC CGTCTACCGATTTCGCCATA 20 20 50.0 50.0 57.7 57.6 0.0 0.0 0.728315 46158 47211 1172-1297 1.000000 81.9 rps4-trnL(UUA)_p5 rps4-trnL(UUA) 5 ATCGCTTCCAGCTTTAGTCC ATTCCGGGGTTTCTCTGGAT 20 20 50.0 50.0 57.7 58.4 0.0 0.0 0.721034 46158 47211 1250-1375 1.000000 81.7 ndhC-trnV(UAC)_p1 ndhC-trnV(UAC) 1 TCATATTCGTAAAGCAAAAACA GTTCGAGTCCGTATAGCCCT 22 20 27.3 55.0 52.7 58.7 0.0 0.0 7.966059 50573 51622 1094-1110 1.000000 49.0 ndhC-trnV(UAC)_p2 ndhC-trnV(UAC) 2 TCATATTCGTAAAGCAAAAACA AGAAGGTCTACGGTTCGAGT 22 20 27.3 50.0 52.7 57.8 0.0 0.0 7.479993 50573 51622 1106-1122 1.000000 48.9 ndhC-trnV(UAC)_p3 ndhC-trnV(UAC) 3 TCATATTCGTAAAGCAAAAACA GAAGGTCTACGGTTCGAGTC 22 20 27.3 55.0 52.7 57.5 0.0 0.0 7.823765 50573 51622 1105-1121 1.000000 48.9 ndhC-trnV(UAC)_p4 ndhC-trnV(UAC) 4 ATCATATTCGTAAAGCAAAAACA AGAAGGTCTACGGTTCGAGT 23 20 26.1 50.0 53.3 57.8 0.0 0.0 7.849206 50573 51622 1107-1123 1.000000 48.9 ndhC-trnV(UAC)_p5 ndhC-trnV(UAC) 5 TCATATTCGTAAAGCAAAAACAT AGAAGGTCTACGGTTCGAGT 23 20 26.1 50.0 53.3 57.8 0.0 0.0 7.849206 50573 51622 1106-1122 1.000000 48.9 petA-psbJ_p1 petA-psbJ 1 CGAAAAGGTTCAATTGGCCG TCCGGATTAGGTTCATCCCT 20 20 50.0 50.0 58.3 57.5 5.4 0.0 0.792539 63591 64603 1031-1071 1.000000 81.8 petA-psbJ_p2 petA-psbJ 2 CGAAAAGGTTCAATTGGCCG CCGGATTAGGTTCATCCCTG 20 20 50.0 55.0 58.3 57.4 5.4 0.0 0.853842 63591 64603 1030-1070 1.000000 81.3 petA-psbJ_p3 petA-psbJ 3 ACGTGTCCAAGGTCTTTTGT TCCGGATTAGGTTCATCCCT 20 20 45.0 50.0 57.6 57.5 0.0 0.0 0.902837 63591 64603 1109-1149 1.000000 80.4 petA-psbJ_p4 petA-psbJ 4 ACGTGTCCAAGGTCTTTTGT CCGGATTAGGTTCATCCCTG 20 20 45.0 55.0 57.6 57.4 0.0 0.0 0.964140 63591 64603 1108-1148 1.000000 79.9 petA-psbJ_p5 petA-psbJ 5 TCCATTACGTGTCCAAGGTC TCCGGATTAGGTTCATCCCT 20 20 50.0 50.0 57.2 57.5 0.0 0.0 1.244474 63591 64603 1115-1155 1.000000 77.6 rrn16_p1 rrn16 1 CGAATCCGCTTTGTCTACGA TGTGAAGCAGTGTCAAACCA 20 20 50.0 45.0 58.1 57.9 0.0 0.0 0.217147 99840 101330 1592-1656 1.000000 88.7 rrn16_p2 rrn16 2 CGAATCCGCTTTGTCTACGA TGTTTTGGGTGTGAAGCAGT 20 20 50.0 45.0 58.1 58.2 0.0 0.0 0.249849 99840 101330 1601-1665 1.000000 88.5 rrn16_p3 rrn16 3 CGAATCCGCTTTGTCTACGA TTTTGGGTGTGAAGCAGTGT 20 20 50.0 45.0 58.1 58.2 0.0 0.0 0.249849 99840 101330 1599-1663 1.000000 88.5 rrn16_p4 rrn16 4 CGAATCCGCTTTGTCTACGA GTTTTGGGTGTGAAGCAGTG 20 20 50.0 50.0 58.1 57.8 0.0 0.0 0.304182 99840 101330 1600-1664 1.000000 88.0 rrn16_p5 rrn16 5 CGCTTTGTCTACGAACAAGC TGTGAAGCAGTGTCAAACCA 20 20 50.0 45.0 57.8 57.9 0.0 0.0 0.334214 99840 101330 1586-1650 1.000000 87.8 ndhF_p1 ndhF 1 ATTATGGACCCGCTACCAGA AAAGAGGTAAGGTCCGGTCA 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.116917 109176 111404 2685-2685 0.333333 52.4 ndhF_p2 ndhF 2 ATTATGGACCCGCTACCAGA AAGAGGTAAGGTCCGGTCAA 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.116917 109176 111404 2684-2684 0.333333 52.4 ndhF_p3 ndhF 3 GGCCCTCCTATCTCTTCCTT AAAGAGGTAAGGTCCGGTCA 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.130560 109176 111404 2907-2907 0.333333 52.3 ndhF_p4 ndhF 4 GGCCCTCCTATCTCTTCCTT AAGAGGTAAGGTCCGGTCAA 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.130560 109176 111404 2906-2906 0.333333 52.3 ndhF_p5 ndhF 5 TATGGACCCGCTACCAGAAA AAGAGGTAAGGTCCGGTCAA 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.151095 109176 111404 2682-2682 0.333333 52.1 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 TGACCGGACCTTACCTCTTT AAAAAGGCCCGAAGTAGCAA 20 20 50.0 45.0 58.0 58.0 9.5 0.0 0.030101 112129 112621 1711-1711 0.333333 53.1 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 TGACCGGACCTTACCTCTTT AAAAGGCCCGAAGTAGCAAA 20 20 50.0 45.0 58.0 58.0 9.5 0.0 0.030101 112129 112621 1710-1710 0.333333 53.1 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 TTGACCGGACCTTACCTCTT AAAAAGGCCCGAAGTAGCAA 20 20 50.0 45.0 58.0 58.0 9.5 0.0 0.030101 112129 112621 1712-1712 0.333333 53.1 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 TTGACCGGACCTTACCTCTT AAAAGGCCCGAAGTAGCAAA 20 20 50.0 45.0 58.0 58.0 9.5 0.0 0.030101 112129 112621 1711-1711 0.333333 53.1 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 TGACCGGACCTTACCTCTTT AGCAAAGCCCTGAGTGAAAA 20 20 50.0 45.0 58.0 57.9 9.5 0.0 0.091585 112129 112621 1696-1696 0.333333 52.6 ndhA_p1 ndhA 1 CGGATTGTTCGTTGACCAGA AGTTGAAGCTCCAAAAGGGG 20 20 50.0 50.0 58.2 58.0 0.0 0.0 0.210321 118113 120257 2364-2511 1.000000 86.2 ndhA_p2 ndhA 2 CTCGGATTGTTCGTTGACCA AGTTGAAGCTCCAAAAGGGG 20 20 50.0 50.0 58.2 58.0 0.0 0.0 0.210321 118113 120257 2366-2513 1.000000 86.2 ndhA_p3 ndhA 3 TCGGATTGTTCGTTGACCAG AGTTGAAGCTCCAAAAGGGG 20 20 50.0 50.0 58.2 58.0 0.0 0.0 0.210321 118113 120257 2365-2512 1.000000 86.2 ndhA_p4 ndhA 4 CGGATTGTTCGTTGACCAGA GTTGAAGCTCCAAAAGGGGA 20 20 50.0 50.0 58.2 58.0 0.0 0.0 0.213869 118113 120257 2363-2510 1.000000 86.1 ndhA_p5 ndhA 5 TCGGATTGTTCGTTGACCAG GTTGAAGCTCCAAAAGGGGA 20 20 50.0 50.0 58.2 58.0 0.0 0.0 0.213869 118113 120257 2364-2511 1.000000 86.1 ycf1_p1 ycf1 1 TCGTCTATATTTATGAGATAAGACTC TGAAAGAAACCTCAAAAACGGA 26 22 30.8 36.4 53.9 56.7 0.0 0.0 13.394264 126870 126989 172-185 1.000000 48.5 ycf1_p2 ycf1 2 TCGTCTATATTTATGAGATAAGACTC ACTGAAAGAAACCTCAAAAACGG 26 23 30.8 39.1 53.9 58.0 0.0 0.0 13.078321 126870 126989 174-187 1.000000 48.4 ycf1_p3 ycf1 3 TCGTCTATATTTATGAGATAAGACT ACTGAAAGAAACCTCAAAAACGG 25 23 28.0 39.1 52.8 58.0 0.0 0.0 13.249881 126870 126989 174-187 1.000000 48.4 ycf1_p4 ycf1 4 TCGTCTATATTTATGAGATAAGACTC CTGAAAGAAACCTCAAAAACGGA 26 23 30.8 39.1 53.9 57.8 0.0 0.0 13.315651 126870 126989 173-186 1.000000 48.4 ycf1_p5 ycf1 5 CGTCTATATTTATGAGATAAGACTC ACTGAAAGAAACCTCAAAAACGG 25 23 32.0 39.1 52.6 58.0 0.0 0.0 13.417753 126870 126989 173-186 1.000000 48.4 rrn16_p1 rrn16 1 TGTGAAGCAGTGTCAAACCA CGAATCCGCTTTGTCTACGA 20 20 45.0 50.0 57.9 58.1 0.0 0.0 0.217147 134870 136360 1592-1656 1.000000 88.5 rrn16_p2 rrn16 2 TGTTTTGGGTGTGAAGCAGT CGAATCCGCTTTGTCTACGA 20 20 45.0 50.0 58.2 58.1 0.0 0.0 0.249849 134870 136360 1601-1665 1.000000 88.3 rrn16_p3 rrn16 3 TTTTGGGTGTGAAGCAGTGT CGAATCCGCTTTGTCTACGA 20 20 45.0 50.0 58.2 58.1 0.0 0.0 0.249849 134870 136360 1599-1663 1.000000 88.3 rrn16_p4 rrn16 4 GTTTTGGGTGTGAAGCAGTG CGAATCCGCTTTGTCTACGA 20 20 50.0 50.0 57.8 58.1 0.0 0.0 0.304182 134870 136360 1600-1664 1.000000 87.8 rrn16_p5 rrn16 5 TGTGAAGCAGTGTCAAACCA CGCTTTGTCTACGAACAAGC 20 20 45.0 50.0 57.9 57.8 0.0 0.0 0.334214 134870 136360 1586-1650 1.000000 87.6