Hotspot

ycf1

SSC · 106129-110583 · 4455 bp

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Pi 0.00898

MarkerSeek score 48.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4455
variable_sites: 115
pi: 0.00898
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCTCTTACTTCGGGAATAACTCA	AATCCCATTTTGATAAATCCG	43.5	33.3	57.4	52.2	166 bp	1.0	44.3
ycf1_p2	ACCTCTTACTTCGGGAATAACTC	AATCCCATTTTGATAAATCCG	43.5	33.3	57.4	52.2	167 bp	1.0	44.3
ycf1_p3	GCTCTTTTTCCTAACGCTAAACT	AATCCCATTTTGATAAATCCG	39.1	33.3	57.4	52.2	244 bp	1.0	43.0
ycf1_p4	AGCTCTTTTTCCTAACGCTA	AATCCCATTTTGATAAATCCG	40.0	33.3	54.4	52.2	245 bp	1.0	42.9
ycf1_p5	TAGCTCTTTTTCCTAACGCT	AATCCCATTTTGATAAATCCG	40.0	33.3	54.4	52.2	246 bp	1.0	42.9