Hotspot

rpl32-ndhF

SSC · 120441-121073 · 633 bp

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Pi 0.0129

MarkerSeek score 59.60

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 633
variable_sites: 25
pi: 0.0129
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TGCCAGAGAAGTACGTTTTT	AGGAGAGGTATGATCCATGCA	40.0	47.6	55.2	58.0	704-725 bp	1.0	53.7
rpl32-ndhF_p2	TTGCCAGAGAAGTACGTTTT	AGGAGAGGTATGATCCATGCA	40.0	47.6	55.2	58.0	705-726 bp	1.0	53.7
rpl32-ndhF_p3	TTTGCCAGAGAAGTACGTTT	AGGAGAGGTATGATCCATGCA	40.0	47.6	55.2	58.0	706-727 bp	1.0	53.7
rpl32-ndhF_p4	TTTTGCCAGAGAAGTACGTT	AGGAGAGGTATGATCCATGCA	40.0	47.6	55.2	58.0	707-728 bp	1.0	53.7
rpl32-ndhF_p5	TTTTTGCCAGAGAAGTACGT	AGGAGAGGTATGATCCATGCA	40.0	47.6	55.2	58.0	708-729 bp	1.0	53.7