Hotspot

trnL-UAG-rpl32

SSC · 119795-120281 · 487 bp

Back to Echeveria

Pi 0.0196

MarkerSeek score 56.40

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 487
variable_sites: 32
pi: 0.0196
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	TTCGAACCGAGATGCTCAAG	TAGGAGGTCACAGTTGGGTT	50.0	50.0	58.0	57.9	1014-1018 bp	1.0	83.9
trnL-UAG-rpl32_p2	CGAAGTGGCAAATGCTTGAG	TAGGAGGTCACAGTTGGGTT	50.0	50.0	58.0	57.9	1439-1443 bp	1.0	82.5
trnL-UAG-rpl32_p3	CTCGAAGTGGCAAATGCTTG	TAGGAGGTCACAGTTGGGTT	50.0	50.0	58.0	57.9	1441-1445 bp	1.0	82.5
trnL-UAG-rpl32_p4	TTCGAACCGAGATGCTCAAG	TTAGGAGGTCACAGTTGGGT	50.0	50.0	58.0	57.9	1018-1019 bp	0.857143	77.8
trnL-UAG-rpl32_p5	CGAAGTGGCAAATGCTTGAG	TTAGGAGGTCACAGTTGGGT	50.0	50.0	58.0	57.9	1443-1444 bp	0.857143	76.6