Hotspot

rpl20-rps12

LSC · 66500-67283 · 784 bp

Back to Echeveria

Pi 0.00562

MarkerSeek score 60.90

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 784
variable_sites: 11
pi: 0.00562
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TTGTTTCTACGTCTCCGAGC	CACAAAATCCCCCGCTCTTA	50.0	50.0	57.7	57.9	885-892 bp	1.0	77.9
rpl20-rps12_p2	TTGTTTCTACGTCTCCGAGC	GCTCTTAGGGGATGTCCTCA	50.0	55.0	57.7	58.2	872-879 bp	1.0	77.3
rpl20-rps12_p3	TTGTTTCTACGTCTCCGAGC	TCTTAGGGGATGTCCTCAGC	50.0	55.0	57.7	58.2	870-877 bp	1.0	77.3
rpl20-rps12_p4	TGTTTCTACGTCTCCGAGCT	CACAAAATCCCCCGCTCTTA	50.0	50.0	58.5	57.9	884-891 bp	1.0	76.9
rpl20-rps12_p5	TTGTTTCTACGTCTCCGAGC	CAAAATCCCCCGCTCTTAGG	50.0	55.0	57.7	58.3	883-890 bp	1.0	76.3