Hotspot

ycf4-cemA

LSC · 58823-59438 · 616 bp

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Pi 0.0126

MarkerSeek score 62.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 616
variable_sites: 20
pi: 0.0126
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGACTCCACGCGAAATTGAA	GGGATCAACCAGGGCAAAAA	45.0	50.0	58.1	58.7	761-782 bp	1.0	76.6
ycf4-cemA_p2	TTGACTCCACGCGAAATTGA	GGGATCAACCAGGGCAAAAA	45.0	50.0	58.1	58.7	762-783 bp	1.0	76.6
ycf4-cemA_p3	AAAAAGCTGCTGAATTGGCC	GGGATCAACCAGGGCAAAAA	45.0	50.0	57.8	58.7	740-761 bp	1.0	76.3
ycf4-cemA_p4	AAAGCTGCTGAATTGGCCTA	GGGATCAACCAGGGCAAAAA	45.0	50.0	57.8	58.7	738-759 bp	1.0	75.9
ycf4-cemA_p5	GACTCCACGCGAAATTGAAC	GGGATCAACCAGGGCAAAAA	50.0	50.0	57.8	58.7	760-781 bp	1.0	75.8