Hotspot

rbcL-accD

LSC · 55179-55907 · 729 bp

Back to Echeveria

Pi 0.00933

MarkerSeek score 63.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 729
variable_sites: 18
pi: 0.00933
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGTCCTGAACTAGCTGCTG	AGCATCGAATTGAACCACCG	55.0	50.0	57.7	58.9	761-836 bp	1.0	76.1
rbcL-accD_p2	GCTCGCGAGGGTAATGAAAT	AGCATCGAATTGAACCACCG	50.0	50.0	58.4	58.9	802-877 bp	1.0	75.1
rbcL-accD_p3	GCTGCTGCTTGTGAAGTATG	AGCATCGAATTGAACCACCG	50.0	50.0	57.5	58.9	748-823 bp	1.0	74.2
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	GCATCGAATTGAACCACCGT	55.0	50.0	57.7	59.2	760-835 bp	1.0	73.9
rbcL-accD_p5	GAGTCCTGAACTAGCTGCTG	CATCGAATTGAACCACCGTT	55.0	45.0	57.7	56.7	759-834 bp	1.0	73.3