Hotspot

petN-psbM

LSC · 29011-29806 · 796 bp

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Pi 0.00634
MarkerSeek score 60.80
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
796
variable_sites
15
pi
0.00634
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 AGTAAGTCTTGCTTGGGCTG TGCTACTGCACTGTTCATTT 50.0 40.0 57.8 55.6 950-965 bp 1.0 60.9
petN-psbM_p2 TCTTGCTTGGGCTGGTTTAA TGCTACTGCACTGTTCATTT 45.0 40.0 57.6 55.6 944-959 bp 1.0 59.5
petN-psbM_p3 GTCTTGCTTGGGCTGGTTTA TGCTACTGCACTGTTCATTT 50.0 40.0 58.4 55.6 945-960 bp 1.0 59.4
petN-psbM_p4 AGTAAGTCTTGCTTGGGCTG TGCTACTGCACTGTTCATTTT 50.0 38.1 57.8 56.4 950-965 bp 1.0 59.2
petN-psbM_p5 TCTTGCTTGGGCTGGTTTAA TGCTACTGCACTGTTCATTTT 45.0 38.1 57.6 56.4 944-959 bp 1.0 57.8