Hotspot

trnG-GCC-trnR-UCU

LSC · 9959-10214 · 256 bp

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Pi 0.111

MarkerSeek score 71.90

Primer available yes

Label trnG-GCC-trnR-UCU

Feature Metadata

label_name: trnG-GCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 256
variable_sites: 43
pi: 0.111
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnR-UCU_p1	TAGCCTTCCAAGCTACCGAT	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	58.2	55.6	289-368 bp	1.0	55.3
trnG-GCC-trnR-UCU_p2	TAGCCTTCCAAGCTACCGAT	AGAAGACCTATGTCCTATCCA	50.0	42.9	58.2	54.6	283-362 bp	1.0	55.1
trnG-GCC-trnR-UCU_p3	TAGCCTTCCAAGCTACCGAT	ACCAAAGGTTTAGAAGACCTATGT	50.0	37.5	58.2	57.6	294-373 bp	1.0	54.7
trnG-GCC-trnR-UCU_p4	TAGCCTTCCAAGCTACCGAT	AAGGTTTAGAAGACCTATGTCCT	50.0	39.1	58.2	56.4	290-369 bp	1.0	53.3
trnG-GCC-trnR-UCU_p5	TAGCCTTCCAAGCTACCGAT	AGAAGACCTATGTCCTATCCAT	50.0	40.9	58.2	55.2	283-362 bp	1.0	52.0