Hotspot

ycf2

Genome · 1-2725 · 2725 bp

Back to Dystaenia

Pi 0.282

MarkerSeek score 66.90

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 2725
variable_sites: 11
pi: 0.282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.01
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	GGAACCATATCACATCCCGG	ACTGTCTACGGTTCGATTGC	55.0	50.0	57.8	58.0	1793 bp	0.5	58.5
ycf2_p2	GGAACCATATCACATCCCGG	GTTGGGCGCTTTAACCATTC	55.0	50.0	57.8	58.0	1226 bp	0.5	58.4
ycf2_p3	CGGAACCATATCACATCCCG	ACTGTCTACGGTTCGATTGC	55.0	50.0	58.2	58.0	1794 bp	0.5	58.3
ycf2_p4	CGGAACCATATCACATCCCG	GTTGGGCGCTTTAACCATTC	55.0	50.0	58.2	58.0	1227 bp	0.5	58.3
ycf2_p5	GGAACCATATCACATCCCGG	TTCTACCCTTTCCCGGAAGT	55.0	50.0	57.8	58.0	1614 bp	0.5	58.2