Hotspot

rpoA

Genome · 10178-11239 · 1062 bp

Back to Dystaenia

Pi 0.143

MarkerSeek score 69.20

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: Genome
length_bp: 1062
variable_sites: 2
pi: 0.143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.01
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TGTAGACCTCCGAAAAAGCG	ACGACCTGTGTATCTAGGGA	50.0	50.0	57.9	56.9	1252 bp	0.5	50.5
rpoA_p2	TGTAGACCTCCGAAAAAGCG	CACGACCTGTGTATCTAGGG	50.0	55.0	57.9	56.8	1253 bp	0.5	50.1
rpoA_p3	CGAAAAAGCGACGTGTGTAG	ACGACCTGTGTATCTAGGGA	50.0	50.0	57.8	56.9	1242 bp	0.5	49.5
rpoA_p4	CGAAAAAGCGACGTGTGTAG	CACGACCTGTGTATCTAGGG	50.0	55.0	57.8	56.8	1243 bp	0.5	49.2
rpoA_p5	TGTAGACCTCCGAAAAAGCG	CACGACCTGTGTATCTAGGGA	50.0	52.4	57.9	58.3	1253 bp	0.5	48.6