Hotspot

rpl16

Genome · 6524-7880 · 1357 bp

Back to Dystaenia

Pi 0.2

MarkerSeek score 69.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: Genome
length_bp: 1357
variable_sites: 2
pi: 0.2
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.01
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGTTCAATCAAAACAAAACCA	AAACCGGCACCTGTTATTGT	28.6	45.0	52.9	58.0	1492 bp	0.5	20.0
rpl16_p2	TGTTCAATCAAAACAAAACCA	AACCGGCACCTGTTATTGTT	28.6	45.0	52.9	58.0	1491 bp	0.5	20.0
rpl16_p3	TGTTCAATCAAAACAAAACCA	ACCGGCACCTGTTATTGTTT	28.6	45.0	52.9	58.0	1490 bp	0.5	20.0
rpl16_p4	TGTTCAATCAAAACAAAACCA	TCCAAAGAAAGAAACCGGCA	28.6	45.0	52.9	57.9	1503 bp	0.5	20.0
rpl16_p5	TGTTCAATCAAAACAAAACCA	AAAGAAAGAAACCGGCACCT	28.6	45.0	52.9	57.9	1500 bp	0.5	20.0