Hotspot

psbH

Genome · 14321-14542 · 222 bp

Back to Dystaenia

Pi NA

MarkerSeek score 35.40

Primer available yes

Label psbH

Feature Metadata

label_name: psbH
feature_type: gene
region: Genome
length_bp: 222
variable_sites: 0
pi: NA
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 35.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH_p1	CGCGGTCGAACTACCAAAAT	ACAAAGTCAACAGCTCTCAATG	50.0	40.9	58.9	57.2	360 bp	0.5	30.0
psbH_p2	CGCGGTCGAACTACCAAAAT	ACAAAGTCAACAGCTCTCAA	50.0	40.0	58.9	55.1	360 bp	0.5	29.6
psbH_p3	CGCGGTCGAACTACCAAAAT	CAAAGTCAACAGCTCTCAATGA	50.0	40.9	58.9	56.9	359 bp	0.5	27.9
psbH_p4	CGCGGTCGAACTACCAAAAT	AGGGTTGCTGTTTCCATTAT	50.0	40.0	58.9	54.9	442 bp	0.5	27.6
psbH_p5	CGCGGTCGAACTACCAAAAT	ACAAAGTCAACAGCTCTCAAT	50.0	38.1	58.9	55.7	360 bp	0.5	26.4