Hotspot

clpP

Genome · 89583-91671 · 2089 bp

Back to Dystaenia

Pi 0.0044

MarkerSeek score 62.00

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: Genome
length_bp: 2089
variable_sites: 9
pi: 0.0044
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GGGCTGGTTTAGATCGATCC	TACGTTTCATCACCACCACC	55.0	50.0	57.8	57.8	2288 bp	0.5	57.2
clpP_p2	GGGCTGGTTTAGATCGATCC	GGATCGACGGGAAGATTTGT	55.0	50.0	57.8	57.7	2316 bp	0.5	56.1
clpP_p3	GGGCTGGTTTAGATCGATCC	CGTTTCATCACCACCACCTT	55.0	50.0	57.8	58.4	2286 bp	0.5	55.5
clpP_p4	GAAATCGAGAATTTGCGCGA	TACGTTTCATCACCACCACC	45.0	50.0	57.6	57.8	2203 bp	0.5	55.1
clpP_p5	GGAAATCGAGAATTTGCGCG	TACGTTTCATCACCACCACC	50.0	50.0	58.5	57.8	2204 bp	0.5	54.3