Hotspot

matK-trnK

Genome · 22923-23668 · 746 bp

Back to Dystaenia

Pi 0.0067

MarkerSeek score 62.50

Primer available yes

Label matK-trnK

Feature Metadata

label_name: matK-trnK
feature_type: igs
region: Genome
length_bp: 746
variable_sites: 5
pi: 0.0067
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK_p1	AACCATGATCATGCGCAAGT	AACGGTAGAGTACTCGGCTT	45.0	50.0	58.5	58.2	879-880 bp	1.0	74.8
matK-trnK_p2	AACCATGATCATGCGCAAGT	ACGGTAGAGTACTCGGCTTT	45.0	50.0	58.5	58.2	878-879 bp	1.0	74.8
matK-trnK_p3	AAACCATGATCATGCGCAAG	AACGGTAGAGTACTCGGCTT	45.0	50.0	57.4	58.2	880-881 bp	1.0	74.5
matK-trnK_p4	AAACCATGATCATGCGCAAG	ACGGTAGAGTACTCGGCTTT	45.0	50.0	57.4	58.2	879-880 bp	1.0	74.5
matK-trnK_p5	AACCATGATCATGCGCAAGT	GGGTTGCTAACTCAACGGTA	45.0	50.0	58.5	57.5	892-893 bp	1.0	72.5