Hotspot

psbB-trnH-GUG

Genome · 16692-19578 · 2887 bp

Back to Dystaenia

Pi 0.087

MarkerSeek score 57.50

Primer available yes

Label psbB-trnH-GUG

Feature Metadata

label_name: psbB-trnH-GUG
feature_type: igs
region: Genome
length_bp: 2887
variable_sites: 2
pi: 0.087
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.01
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-trnH-GUG_p1	CGATACCAAGGCAAACCCAT	CGGATGTAGCCAAGTGGATC	50.0	55.0	58.2	58.1	2980 bp	0.5	57.1
psbB-trnH-GUG_p2	CGATACCAAGGCAAACCCAT	TGGATCAAGGCAGTGGATTG	50.0	50.0	58.2	57.9	2966 bp	0.5	57.0
psbB-trnH-GUG_p3	CGATACCAAGGCAAACCCAT	GGATCAAGGCAGTGGATTGT	50.0	50.0	58.2	57.9	2965 bp	0.5	57.0
psbB-trnH-GUG_p4	CGATACCAAGGCAAACCCAT	GTGGATCAAGGCAGTGGATT	50.0	50.0	58.2	57.9	2967 bp	0.5	57.0
psbB-trnH-GUG_p5	ACACGATACCAAGGCAAACC	CGGATGTAGCCAAGTGGATC	50.0	55.0	58.5	58.1	2983 bp	0.5	55.2