Hotspot

rrn4.5-rrn23

IRa · 129833-129937 · 105 bp

Back to Dysphania

Pi 0.216

MarkerSeek score 85.30

Primer available yes

Label rrn4.5-rrn23

Feature Metadata

label_name: rrn4.5-rrn23
feature_type: igs
region: IRa
length_bp: 105
variable_sites: 32
pi: 0.216
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5-rrn23_p1	CTGTGGGTCTGTTAGGATGC	AAGTAGTAAGCCCACCCCAA	55.0	50.0	58.0	58.2	236-283 bp	1.0	89.8
rrn4.5-rrn23_p2	TCACTGCACTTCCACTTGAC	AAGTAGTAAGCCCACCCCAA	50.0	50.0	58.0	58.2	202-249 bp	1.0	89.6
rrn4.5-rrn23_p3	CTGTTAGGATGCCTCAGCTG	AAGTAGTAAGCCCACCCCAA	55.0	50.0	58.0	58.2	228-275 bp	1.0	89.6
rrn4.5-rrn23_p4	GTCTGTTAGGATGCCTCAGC	AAGTAGTAAGCCCACCCCAA	55.0	50.0	58.1	58.2	230-277 bp	1.0	89.6
rrn4.5-rrn23_p5	CTGTGGGTCTGTTAGGATGC	TAGTAAGCCCACCCCAAGAT	55.0	50.0	58.0	57.7	233-280 bp	1.0	89.5