Hotspot

rpl32-trnL-UAG

SSC · 111884-112940 · 1057 bp

Back to Dysphania

Pi 0.0824
MarkerSeek score 84.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1057
variable_sites
124
pi
0.0824
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGGGCAGCATTAAAAGCCTT CTCGCACTGCTTCCTAAGAG 45.0 55.0 58.4 58.1 1157-1185 bp 0.666667 73.9
rpl32-trnL-UAG_p2 TGGGCAGCATTAAAAGCCTT CTCTCGCACTGCTTCCTAAG 45.0 55.0 58.4 58.1 1159-1187 bp 0.666667 73.9
rpl32-trnL-UAG_p3 TTGGGCAGCATTAAAAGCCT CTCGCACTGCTTCCTAAGAG 45.0 55.0 58.4 58.1 1158-1186 bp 0.666667 73.9
rpl32-trnL-UAG_p4 TGGGCAGCATTAAAAGCCTT CCGCCTAAAAGAGCTGCATA 45.0 50.0 58.4 58.0 1772-1813 bp 0.666667 72.4
rpl32-trnL-UAG_p5 TTGGGCAGCATTAAAAGCCT CCGCCTAAAAGAGCTGCATA 45.0 50.0 58.4 58.0 1773-1814 bp 0.666667 72.4