Hotspot

psbE-petL

LSC · 63819-64942 · 1124 bp

Back to Dysphania

Pi 0.0553

MarkerSeek score 84.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1124
variable_sites: 92
pi: 0.0553
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAAAAACCCG	50.0	50.0	57.9	58.6	1266-1277 bp	1.0	81.4
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GCCGCTAGTAAAAACCCGAA	50.0	50.0	57.9	58.6	1264-1275 bp	1.0	81.4
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAAAAACCCG	50.0	50.0	57.8	58.6	1189-1200 bp	1.0	80.9
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAAAAACCCGAA	50.0	50.0	57.8	58.6	1187-1198 bp	1.0	80.9
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AAGCCGCTAGTAAAAACCCG	45.0	50.0	58.2	58.6	1275-1286 bp	1.0	80.4