Hotspot

trnT-GGU-psbD

LSC · 30596-31762 · 1167 bp

Back to Dysphania

Pi 0.0389
MarkerSeek score 78.50
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1167
variable_sites
66
pi
0.0389
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG CGTAACCAGTCATCCATACT 55.0 45.0 58.1 54.3 1296-1354 bp 1.0 57.1
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA CGTAACCAGTCATCCATACT 50.0 45.0 58.1 54.3 1299-1357 bp 1.0 56.9
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA CGTAACCAGTCATCCATACT 50.0 45.0 58.3 54.3 1273-1331 bp 1.0 55.5
trnT-GGU-psbD_p4 GGCCCTTTTAACTCAGTGGT CGTAACCAGTCATCCATACT 50.0 45.0 57.7 54.3 1311-1369 bp 1.0 55.2
trnT-GGU-psbD_p5 TGGTAGAGTAACGCCATGGT CGTAACCAGTCATCCATACT 50.0 45.0 58.4 54.3 1295-1353 bp 1.0 54.1