Hotspot

trnD-GUC-trnY-GUA

LSC · 29372-29790 · 419 bp

Back to Dysphania

Pi 0.0636

MarkerSeek score 82.70

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 419
variable_sites: 39
pi: 0.0636
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GCGGTGCTCTAACCAATTGA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.6	57.8	490-501 bp	1.0	80.8
trnD-GUC-trnY-GUA_p2	GCGGTGCTCTAACCAATTGA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.6	57.8	489-500 bp	1.0	80.8
trnD-GUC-trnY-GUA_p3	GCGGTGCTCTAACCAATTGA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.6	58.4	511-522 bp	1.0	79.6
trnD-GUC-trnY-GUA_p4	GCGGTGCTCTAACCAATTGA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.6	57.6	510-521 bp	1.0	79.4
trnD-GUC-trnY-GUA_p5	TGATTATTGGATCCGTCGGG	TGGCAATATGTCTACGCTGG	50.0	50.0	57.2	57.8	549-560 bp	1.0	77.8