Hotspot

rpoB-trnC-GCA

LSC · 26083-27042 · 960 bp

Back to Dysphania

Pi 0.0512

MarkerSeek score 80.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 960
variable_sites: 69
pi: 0.0512
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGAGCCCATATCCTTGCTTT	GGGGGTTCGAGTCTTTTGTT	50.0	50.0	58.0	58.0	1573-1733 bp	1.0	87.5
rpoB-trnC-GCA_p2	CCCGGGCTTTGCAATATTTG	GGGGGTTCGAGTCTTTTGTT	50.0	50.0	58.1	58.0	1453-1613 bp	1.0	87.5
rpoB-trnC-GCA_p3	GAAAATACCGGATCCCCACC	GGGGGTTCGAGTCTTTTGTT	55.0	50.0	58.0	58.0	1780-1940 bp	1.0	87.2
rpoB-trnC-GCA_p4	CGAGCCCATATCCTTGCTTT	CGGATTTGAACTGGGGAACA	50.0	50.0	58.0	58.1	1533-1693 bp	1.0	87.0
rpoB-trnC-GCA_p5	GAAAATACCGGATCCCCACC	CGGATTTGAACTGGGGAACA	55.0	50.0	58.0	58.1	1740-1900 bp	1.0	86.6