Hotspot

trnS-GCU-trnR-UCU

LSC · 7797-9421 · 1625 bp

Back to Dysphania

Pi 0.0417

MarkerSeek score 78.40

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1625
variable_sites: 99
pi: 0.0417
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	ACGGAAAGAGAGGGATTCGA	AGAAGACCCATGTCCTATCCA	50.0	47.6	58.2	57.6	1747-1750 bp	1.0	73.1
trnS-GCU-trnR-UCU_p2	CGAATGACTCGTACAACGGA	AGAAGACCCATGTCCTATCCA	50.0	47.6	57.7	57.6	1717-1720 bp	1.0	72.4
trnS-GCU-trnR-UCU_p3	CTTTCGTCCACTCAGCCATC	AGAAGACCCATGTCCTATCCA	55.0	47.6	58.6	57.6	1683-1686 bp	1.0	69.4
trnS-GCU-trnR-UCU_p4	AACGGAAAGAGAGGGATTCG	AGAAGACCCATGTCCTATCCA	50.0	47.6	57.3	57.6	1748-1751 bp	1.0	68.9
trnS-GCU-trnR-UCU_p5	ACGGAAAGAGAGGGATTCGA	GAAGACCCATGTCCTATCCA	50.0	50.0	58.2	56.0	1746-1749 bp	1.0	68.5