Hotspot

trnK-UUU-rps16

LSC · 4075-4636 · 562 bp

Back to Dysphania

Pi 0.0626
MarkerSeek score 83.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
562
variable_sites
51
pi
0.0626
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TCGACTTAATCAAATGAAGTTCA 50.0 30.4 58.2 54.3 603-629 bp 1.0 49.3
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TCGACTTAATCAAATGAAGTTCA 50.0 30.4 58.2 54.3 602-628 bp 1.0 49.3
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT TCGACTTAATCAAATGAAGTTCAA 50.0 29.2 58.2 55.1 602-628 bp 1.0 49.3
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA TCGACTTAATCAAATGAAGTTCA 50.0 30.4 58.3 54.3 612-638 bp 1.0 49.2
trnK-UUU-rps16_p5 TCTAGCCGCACTTAAAAGCC TCGACTTAATCAAATGAAGTTCA 50.0 30.4 58.3 54.3 617-643 bp 1.0 49.1