Hotspot

psbA-trnK-UUU

LSC · 1277-1489 · 213 bp

Back to Dysphania

Pi 0.0485

MarkerSeek score 78.30

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 213
variable_sites: 15
pi: 0.0485
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	ATAGGCTTTCACTTTCGCGT	CTCCATCCGATTGGTTCCG	45.0	57.9	57.9	58.0	285-294 bp	1.0	77.8
psbA-trnK-UUU_p2	ATAGGCTTTCACTTTCGCGT	CTACTCCATCCGATTGGTTCC	45.0	52.4	57.9	57.9	288-297 bp	1.0	76.9
psbA-trnK-UUU_p3	ATAGGCTTTCACTTTCGCGT	GATTGGTTCCGGGTTCGAG	45.0	57.9	57.9	58.2	277-286 bp	1.0	76.1
psbA-trnK-UUU_p4	ATAGGCTTTCACTTTCGCGT	TACTCCATCCGATTGGTTCC	45.0	50.0	57.9	56.7	287-296 bp	1.0	75.4
psbA-trnK-UUU_p5	CATAGGCTTTCACTTTCGCG	CTCCATCCGATTGGTTCCG	50.0	57.9	57.6	58.0	286-295 bp	1.0	75.0