Hotspot

rrn16

IRa · 139733-141223 · 1491 bp

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Pi 0.0427
MarkerSeek score 64.60
Primer available yes
Label rrn16

Feature Metadata

label_name
rrn16
feature_type
rRNA
region
IRa
length_bp
1491
variable_sites
127
pi
0.0427
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn16_p1 GGCTCCAAGTTGTTCAGGAA TGAATCGCCCCATCTATCCT 50.0 50.0 58.0 58.0 2390-2393 bp 1.0 86.2
rrn16_p2 GGCTCCAAGTTGTTCAGGAA CAGACGTAGCTCGCTTCTTT 50.0 50.0 58.0 58.0 1962 bp 0.75 69.8
rrn16_p3 GAGCAAACTCCAGGCGAATA CAGACGTAGCTCGCTTCTTT 50.0 50.0 58.0 58.0 1682 bp 0.75 69.7
rrn16_p4 GCAAGAAGAAGGCCGTATCA CAGACGTAGCTCGCTTCTTT 50.0 50.0 58.0 58.0 2246 bp 0.75 69.7
rrn16_p5 GAGCAAACTCCAGGCGAATA TGAATCGCCCCATCTATCCT 50.0 50.0 58.0 58.0 2113 bp 0.75 69.6
rrn16_p1 TGAATCGCCCCATCTATCCT GAGCAAACTCCAGGCGAATA 50.0 50.0 58.0 58.0 2110-2113 bp 1.0 86.2
rrn16_p2 CAGACGTAGCTCGCTTCTTT GGCTCCAAGTTGTTCAGGAA 50.0 50.0 58.0 58.0 1962 bp 0.75 69.8
rrn16_p3 CAGACGTAGCTCGCTTCTTT GAGCAAACTCCAGGCGAATA 50.0 50.0 58.0 58.0 1682 bp 0.75 69.7
rrn16_p4 CAGACGTAGCTCGCTTCTTT GCAAGAAGAAGGCCGTATCA 50.0 50.0 58.0 58.0 2246 bp 0.75 69.7
rrn16_p5 TGAATCGCCCCATCTATCCT GGCTCCAAGTTGTTCAGGAA 50.0 50.0 58.0 58.0 2393 bp 0.75 69.6