Hotspot

ndhB

IRa · 144201-146436 · 2236 bp

Back to Dysosma

Pi 0.0244

MarkerSeek score 50.60

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: IRa
length_bp: 2236
variable_sites: 109
pi: 0.0244
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	CTGTGGAAAGCCGTATTCGA	TGTAACGACGTAGGGATCCA	50.0	50.0	58.0	57.9	2965-2973 bp	1.0	83.1
ndhB_p2	TCTGTGGAAAGCCGTATTCG	TGTAACGACGTAGGGATCCA	50.0	50.0	58.0	57.9	2966-2974 bp	1.0	83.1
ndhB_p3	CTGTGGAAAGCCGTATTCGA	GTTCATAACCCCAGCCCATT	50.0	50.0	58.0	57.8	2802-2817 bp	1.0	82.8
ndhB_p4	CTGTGGAAAGCCGTATTCGA	TTACATACGCTCGAATGCCC	50.0	50.0	58.0	58.1	2933 bp	0.75	68.9
ndhB_p5	TCTGTGGAAAGCCGTATTCG	TTACATACGCTCGAATGCCC	50.0	50.0	58.0	58.1	2934 bp	0.75	68.9