Hotspot

trnV-GAC-rps12

IRa · 141523-143040 · 1518 bp

Back to Dysosma

Pi 0.0335

MarkerSeek score 72.20

Primer available yes

Label trnV-GAC-rps12

Feature Metadata

label_name: trnV-GAC-rps12
feature_type: igs
region: IRa
length_bp: 1518
variable_sites: 101
pi: 0.0335
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rps12_p1	CCTCAGCCTACGGGGTATTA	TACTCCGACAGCATCTAGGG	55.0	55.0	58.0	58.0	2156-2205 bp	0.75	71.5
trnV-GAC-rps12_p2	CCTCAGCCTACGGGGTATTA	CGAATACGGCTTTCCACAGA	55.0	50.0	58.0	58.0	2657-2706 bp	0.75	71.3
trnV-GAC-rps12_p3	CCTCAGCCTACGGGGTATTA	TCGAATACGGCTTTCCACAG	55.0	50.0	58.0	58.0	2658-2707 bp	0.75	71.3
trnV-GAC-rps12_p4	CATTGCGGAAAATTCCCCAC	CGAATACGGCTTTCCACAGA	50.0	50.0	58.0	58.0	2831-2880 bp	0.75	71.2
trnV-GAC-rps12_p5	CATTGCGGAAAATTCCCCAC	TCGAATACGGCTTTCCACAG	50.0	50.0	58.0	58.0	2832-2881 bp	0.75	71.2