Hotspot

trnN-GUU-trnR-ACG

IRa · 132594-133700 · 1107 bp

Back to Dysosma

Pi 0.00257

MarkerSeek score 65.20

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 1107
variable_sites: 5
pi: 0.00257
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	1189-1248 bp	1.0	82.5
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	1226-1285 bp	1.0	81.8
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	1140-1199 bp	1.0	81.4
trnN-GUU-trnR-ACG_p4	CCGTTGTTCCTCAGTAGCTC	TGAGGGTACTCTTGGGAACA	55.0	50.0	58.0	57.6	1241-1300 bp	1.0	80.7
trnN-GUU-trnR-ACG_p5	TGGTCGTAGGTTCGAATCCT	GAACAGATCCAGTGGAGACG	50.0	55.0	58.2	57.7	1177-1236 bp	1.0	80.7