Hotspot

trnR-ACG-trnN-GUU

IRb · 110470-111576 · 1107 bp

Back to Dysosma

Pi 0.00257

MarkerSeek score 65.20

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 1107
variable_sites: 5
pi: 0.00257
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	1189-1248 bp	1.0	82.5
trnR-ACG-trnN-GUU_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	1226-1285 bp	1.0	81.8
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	1140-1199 bp	1.0	81.4
trnR-ACG-trnN-GUU_p4	TGAGGGTACTCTTGGGAACA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	57.6	58.0	1241-1300 bp	1.0	80.7
trnR-ACG-trnN-GUU_p5	GAACAGATCCAGTGGAGACG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.7	58.2	1177-1236 bp	1.0	80.7