Hotspot

psbC-trnS-UGA

LSC · 36992-37243 · 252 bp

Back to Dysosma

Pi 0.037
MarkerSeek score 69.70
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
252
variable_sites
16
pi
0.037
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 TCCTATTCGTGGGCCATTTG GGTTGATAGCTCCGGTCTTG 50.0 55.0 57.9 58.1 445 bp 1.0 82.0
psbC-trnS-UGA_p2 TCCTATTCGTGGGCCATTTG GATGGCCGAGTGGTTGATAG 50.0 55.0 57.9 58.1 456 bp 1.0 81.4
psbC-trnS-UGA_p3 TCCTATTCGTGGGCCATTTG GGGTTCGAATCCCTCTCTCT 50.0 55.0 57.9 58.2 391 bp 1.0 81.1
psbC-trnS-UGA_p4 CCTATTCGTGGGCCATTTGT GGTTGATAGCTCCGGTCTTG 50.0 55.0 58.2 58.1 444 bp 1.0 80.5
psbC-trnS-UGA_p5 CCTATTCGTGGGCCATTTGT GATGGCCGAGTGGTTGATAG 50.0 55.0 58.2 58.1 455 bp 1.0 79.9