Hotspot

atpF-atpH

LSC · 12788-13283 · 496 bp

Back to Dysosma

Pi 0.0104

MarkerSeek score 66.30

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 496
variable_sites: 10
pi: 0.0104
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TTAAATCCGAAACTCCCGGC	CGGAGGCAGAGGGTAAAATC	50.0	55.0	58.3	58.0	673-695 bp	1.0	80.0
atpF-atpH_p2	TTAAATCCGAAACTCCCGGC	GAGGCAGAGGGTAAAATCCG	50.0	55.0	58.3	58.0	671-693 bp	1.0	80.0
atpF-atpH_p3	TTAAATCCGAAACTCCCGGC	GCAGAGGGTAAAATCCGAGG	50.0	55.0	58.3	58.0	668-690 bp	1.0	80.0
atpF-atpH_p4	TTAAATCCGAAACTCCCGGC	GGCAGAGGGTAAAATCCGAG	50.0	55.0	58.3	58.0	669-691 bp	1.0	80.0
atpF-atpH_p5	CCCAGGGAGACGAAAGAATC	GCAGAGGGTAAAATCCGAGG	55.0	55.0	57.7	58.0	635-657 bp	1.0	79.7