Hotspot

trnK-UUU-rps16

LSC · 4503-5048 · 546 bp

Back to Dysosma

Pi 0.0151
MarkerSeek score 71.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
546
variable_sites
16
pi
0.0151
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.89
markerseek_score
71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCTACCAACGGTATGGACGA TTCTTGAAAAGGGCGCTCAA 50.0 45.0 57.9 58.3 673-982 bp 1.0 82.1
trnK-UUU-rps16_p2 TCTACCAACGGTATGGACGA TTTCTTGAAAAGGGCGCTCA 50.0 45.0 57.9 58.3 674-983 bp 1.0 82.1
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TTCTTGAAAAGGGCGCTCAA 50.0 45.0 58.2 58.3 608-917 bp 1.0 82.0
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TTCTTGAAAAGGGCGCTCAA 50.0 45.0 58.2 58.3 607-916 bp 1.0 82.0
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TTTCTTGAAAAGGGCGCTCA 50.0 45.0 58.2 58.3 608-917 bp 1.0 82.0