Hotspot

psbA

LSC · 129-1190 · 1062 bp

Back to Dypsis

Pi 0.00245
MarkerSeek score 45.60
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
6
pi
0.00245
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 ACAATGGAGCAATACCCAACT GTGCTTGGGAGTCCTTGAAA 42.9 50.0 57.5 58.0 1192 bp 0.4 44.3
psbA_p2 ACAATGGAGCAATACCCAACT GGGAGGGATCCTTTTCCTCT 42.9 55.0 57.5 58.1 1452 bp 0.4 43.8
psbA_p3 ACAATGGAGCAATACCCAACT CTTTCTTCTCGGGACCCAAG 42.9 55.0 57.5 57.9 1527 bp 0.4 43.8
psbA_p4 ACAATGGAGCAATACCCAACT GGTTTGGTGACGTGATGAGA 42.9 50.0 57.5 57.8 1667 bp 0.4 43.3
psbA_p5 ACAATGGAGCAATACCCAACT GTGCTAGAACTTTGGCTCGT 42.9 50.0 57.5 58.2 1884 bp 0.4 42.9