Hotspot

ndhA

SSC · 123522-125663 · 2142 bp

Pi 0.00472

MarkerSeek score 61.20

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2142
variable_sites: 22
pi: 0.00472
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTCACCATAGAGAACA	GTCCACCCGGTTTCATCAAT	47.6	50.0	58.1	58.2	2372-2389 bp	1.0	70.9
ndhA_p2	TGAACCCAGTCACCATAGAGA	GTCCACCCGGTTTCATCAAT	47.6	50.0	57.8	58.2	2375-2392 bp	1.0	70.2
ndhA_p3	ATTCATGAACCCAGTCACCA	GTCCACCCGGTTTCATCAAT	45.0	50.0	56.7	58.2	2380-2397 bp	1.0	69.5
ndhA_p4	TTCATGAACCCAGTCACCAT	GTCCACCCGGTTTCATCAAT	45.0	50.0	56.7	58.2	2379-2396 bp	1.0	69.5
ndhA_p5	AATTCATGAACCCAGTCACCA	GTCCACCCGGTTTCATCAAT	42.9	50.0	57.5	58.2	2381-2398 bp	1.0	67.5