Hotspot

psaC-ndhE

SSC · 120998-121520 · 523 bp

Back to Dypsis

Pi 0.0175
MarkerSeek score 65.10
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
523
variable_sites
19
pi
0.0175
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 CAGGCTCGTACACATTGAGT CTGAAGCAGCTATTGGGCTA 50.0 50.0 57.6 57.4 662-697 bp 1.0 77.7
psaC-ndhE_p2 CAGGCTCGTACACATTGAGT TGAAGCAGCTATTGGGCTAG 50.0 50.0 57.6 57.4 661-696 bp 1.0 77.7
psaC-ndhE_p3 CAGGCTCGTACACATTGAGT TGTTATAGCTGTTGCGGCTG 50.0 50.0 57.6 58.6 682-717 bp 1.0 77.5
psaC-ndhE_p4 CAGGCTCGTACACATTGAGT GAAGCAGCTATTGGGCTAGC 50.0 55.0 57.6 58.8 660-695 bp 1.0 76.6
psaC-ndhE_p5 CAGGCTCGTACACATTGAGT TTTGTTATAGCTGTTGCGGC 50.0 45.0 57.6 57.1 684-719 bp 1.0 75.2