Hotspot

ndhD-psaC

SSC · 120631-120751 · 121 bp

Back to Dypsis

Pi 0.043
MarkerSeek score 74.20
Primer available yes
Label ndhD-psaC

Feature Metadata

label_name
ndhD-psaC
feature_type
igs
region
SSC
length_bp
121
variable_sites
9
pi
0.043
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-psaC_p1 AAATTGAGGAACCCGCGAAT AACCGAGGACTGTGTAGGTT 45.0 50.0 58.2 58.3 300 bp 1.0 78.8
ndhD-psaC_p2 AAATTGAGGAACCCGCGAAT AAGAGATGTGAATCCGCCTG 45.0 50.0 58.2 57.7 278 bp 1.0 78.7
ndhD-psaC_p3 TTGAGGAACCCGCGAATATC AACCGAGGACTGTGTAGGTT 50.0 50.0 57.8 58.3 297 bp 1.0 78.3
ndhD-psaC_p4 TTGAGGAACCCGCGAATATC AAGAGATGTGAATCCGCCTG 50.0 50.0 57.8 57.7 275 bp 1.0 78.2
ndhD-psaC_p5 AAATTGAGGAACCCGCGAAT AGAACCGAGGACTGTGTAGG 45.0 55.0 58.2 58.5 302 bp 1.0 77.4