Hotspot

rpl32-trnL-UAG

SSC · 116999-117787 · 789 bp

Back to Dypsis

Pi 0.015
MarkerSeek score 58.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
789
variable_sites
24
pi
0.015
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTTTTGTGCGACAAACAAAT AGCGTGTCTACCGATTTCAC 30.0 50.0 53.4 58.0 854-864 bp 1.0 45.6
rpl32-trnL-UAG_p2 TTTTTGTGCGACAAACAAAT CAGCGTGTCTACCGATTTCA 30.0 50.0 53.4 58.0 855-865 bp 1.0 45.5
rpl32-trnL-UAG_p3 TTTTTGTGCGACAAACAAAT TCCTAAGAGCAGCGTGTCTA 30.0 50.0 53.4 57.9 864-874 bp 1.0 44.6
rpl32-trnL-UAG_p4 TTTTTGTGCGACAAACAAAT CCTAAGAGCAGCGTGTCTAC 30.0 55.0 53.4 57.8 863-873 bp 1.0 44.0
rpl32-trnL-UAG_p5 TTTTTGTGCGACAAACAAAT CTAAGAGCAGCGTGTCTACC 30.0 55.0 53.4 57.8 862-872 bp 1.0 44.0