Hotspot

ndhF-rpl32

SSC · 116455-116824 · 370 bp

Back to Dypsis

Pi 0.0228
MarkerSeek score 69.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
370
variable_sites
16
pi
0.0228
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTGTTGCTGTCGGAATAAGT GGTGGAAACCGATTTCGCTA 40.0 50.0 55.3 58.3 571-608 bp 1.0 62.4
ndhF-rpl32_p2 TTTGTTGCTGTCGGAATAAGT GGTGGAAACCGATTTCGCTA 38.1 50.0 56.1 58.3 572-609 bp 1.0 60.8
ndhF-rpl32_p3 TTTTGTTGCTGTCGGAATAAGT GGTGGAAACCGATTTCGCTA 36.4 50.0 56.8 58.3 573-610 bp 1.0 58.6
ndhF-rpl32_p4 TTTTTGTTGCTGTCGGAATAAGT GGTGGAAACCGATTTCGCTA 34.8 50.0 57.5 58.3 574-611 bp 1.0 55.9
ndhF-rpl32_p5 TGTTGCTGTCGGAATAAGTA GGTGGAAACCGATTTCGCTA 40.0 50.0 54.4 58.3 570-607 bp 1.0 55.4