Hotspot

rpl22-rps19

IRb · 87104-87250 · 147 bp

Back to Dypsis

Pi 0.049
MarkerSeek score 76.30
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
IRb
length_bp
147
variable_sites
13
pi
0.049
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 GCGAACCAAAAGGAACGATG TCTGACTTTCGCGAGACATG 50.0 50.0 58.0 58.0 698-719 bp 1.0 84.3
rpl22-rps19_p2 GCGAACCAAAAGGAACGATG GTTATCCTGCGCTTGGAAGA 50.0 50.0 58.0 58.0 1262-1283 bp 1.0 82.3
rpl22-rps19_p3 TGCTGCTGCAGAATAAACCA GGGTAGAGCCGGATCTAAGT 45.0 55.0 58.1 58.0 1084-1105 bp 1.0 82.3
rpl22-rps19_p4 GCGAACCAAAAGGAACGATG GGGTAGAGCCGGATCTAAGT 50.0 55.0 58.0 58.0 1399-1420 bp 1.0 82.2
rpl22-rps19_p5 GCGAACCAAAAGGAACGATG GAGCCGGATCTAAGTGTTGG 50.0 55.0 58.0 58.1 1394-1415 bp 1.0 81.7