Hotspot

petA-psbJ

LSC · 65508-66533 · 1026 bp

Back to Dypsis

Pi 0.00643
MarkerSeek score 63.90
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1026
variable_sites
16
pi
0.00643
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC GGTTCATATTCCGGGTTGGG 50.0 55.0 58.0 58.3 1175-1182 bp 1.0 78.5
petA-psbJ_p2 ATCCATTACGTGTCCAAGGC CATATTCCGGGTTGGGTTCA 50.0 50.0 58.0 57.6 1171-1178 bp 1.0 77.6
petA-psbJ_p3 GGCCTTTTGTTCTTCTTGGC GGTTCATATTCCGGGTTGGG 50.0 55.0 57.8 58.3 1158-1165 bp 1.0 77.6
petA-psbJ_p4 ATCCATTACGTGTCCAAGGC GTTCATATTCCGGGTTGGGT 50.0 50.0 58.0 57.6 1174-1181 bp 1.0 77.5
petA-psbJ_p5 CATTACGTGTCCAAGGCCTT GGTTCATATTCCGGGTTGGG 50.0 55.0 58.2 58.3 1172-1179 bp 1.0 77.4