Hotspot

accD-psaI

LSC · 61477-62392 · 916 bp

Back to Dypsis

Pi 0.0165
MarkerSeek score 61.70
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
916
variable_sites
26
pi
0.0165
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 CCACGGTTTCTTTCCCTTGA GAGGGTAAGTTGAGATCTGTCA 50.0 45.5 58.0 56.9 906-1045 bp 1.0 61.3
accD-psaI_p2 TCCACGGTTTCTTTCCCTTG GAGGGTAAGTTGAGATCTGTCA 50.0 45.5 58.0 56.9 907-1046 bp 1.0 61.3
accD-psaI_p3 TCAGCTCCACGGTTTCTTTC GAGGGTAAGTTGAGATCTGTCA 50.0 45.5 58.1 56.9 912-1051 bp 1.0 60.6
accD-psaI_p4 CCACGGTTTCTTTCCCTTGA AGGGTAAGTTGAGATCTGTCA 50.0 42.9 58.0 55.7 905-1044 bp 1.0 59.7
accD-psaI_p5 TCCACGGTTTCTTTCCCTTG AGGGTAAGTTGAGATCTGTCA 50.0 42.9 58.0 55.7 906-1045 bp 1.0 59.7