Hotspot

psaA-ycf3

LSC · 43005-43647 · 643 bp

Back to Dypsis

Pi 0.00399
MarkerSeek score 61.70
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
643
variable_sites
5
pi
0.00399
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 CCCATTCCTCGAAAGACGTT TTGAAGATCACGAGGCGTTT 50.0 45.0 57.9 57.8 754-755 bp 1.0 78.6
psaA-ycf3_p2 CCCATTCCTCGAAAGACGTT TTGGTTGAAGATCACGAGGC 50.0 50.0 57.9 58.2 758-759 bp 1.0 78.3
psaA-ycf3_p3 CCCATTCCTCGAAAGACGTT ATCACGAGGCGTTTCGAATT 50.0 45.0 57.9 58.3 748-749 bp 1.0 77.7
psaA-ycf3_p4 CCCATTCCTCGAAAGACGTT TGAAGATCACGAGGCGTTTC 50.0 50.0 57.9 58.3 753-754 bp 1.0 77.6
psaA-ycf3_p5 CCCATTCCTCGAAAGACGTT GCTATAGCGCTTACTCCAGG 50.0 55.0 57.9 57.6 799-800 bp 1.0 77.0