Hotspot

trnT-GGU-psbD

LSC · 33024-34087 · 1064 bp

Back to Dypsis

Pi 0.00395
MarkerSeek score 64.20
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1064
variable_sites
10
pi
0.00395
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 AACGGGCCCTTTTAACTCAG ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1213-1235 bp 1.0 79.5
trnT-GGU-psbD_p2 GCCCTTTTAACTCAGCGGTA ACAAAAACGAAACGGTCCCT 50.0 45.0 57.9 58.2 1208-1230 bp 1.0 79.4
trnT-GGU-psbD_p3 AACGGGCCCTTTTAACTCAG CCCTCCGTAACCAATCATCC 50.0 55.0 58.1 57.7 1197-1219 bp 1.0 79.4
trnT-GGU-psbD_p4 GCCCTTTTAACTCAGCGGTA CCCTCCGTAACCAATCATCC 50.0 55.0 57.9 57.7 1192-1214 bp 1.0 79.4
trnT-GGU-psbD_p5 AACGGGCCCTTTTAACTCAG GCCAGACCAGCCTACAAAAA 50.0 50.0 58.1 58.4 1226-1248 bp 1.0 78.4