Hotspot

rpl16

LSC · 90029-91690 · 1662 bp

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Pi 0.00199
MarkerSeek score 64.90
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1662
variable_sites
7
pi
0.00199
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 AAAAGGAAACCCGCAATTGT TGAGCAGTTCTAAATTCTATAAGGT 40.0 32.0 56.7 55.8 1728-1785 bp 0.8 35.8
rpl16_p2 AAAGGAAACCCGCAATTGTT TGAGCAGTTCTAAATTCTATAAGGT 40.0 32.0 56.7 55.8 1727-1784 bp 0.8 35.8
rpl16_p3 AAGGAAACCCGCAATTGTTT TGAGCAGTTCTAAATTCTATAAGGT 40.0 32.0 56.7 55.8 1726-1783 bp 0.8 35.8
rpl16_p4 AGGAAACCCGCAATTGTTTT TGAGCAGTTCTAAATTCTATAAGGT 40.0 32.0 56.7 55.8 1725-1782 bp 0.8 35.8
rpl16_p5 AGGAAACCCGCAATTGTTTTT TGAGCAGTTCTAAATTCTATAAGGT 38.1 32.0 57.4 55.8 1725-1782 bp 0.8 35.8