Hotspot

ndhF

SSC · 119486-121735 · 2250 bp

Pi 0.00587

MarkerSeek score 55.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2250
variable_sites: 33
pi: 0.00587
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GAGCTACTGAGGAACAACGG	AGAGCCGGTGAATCAGAAAC	55.0	50.0	58.0	57.9	2724-2892 bp	1.0	85.5
ndhF_p2	CCCGGAACTTCTTCGTAGTG	AGAGCCGGTGAATCAGAAAC	55.0	50.0	58.0	57.9	2628-2797 bp	1.0	85.5
ndhF_p3	CCGGAACTTCTTCGTAGTGG	AGAGCCGGTGAATCAGAAAC	55.0	50.0	58.0	57.9	2627-2796 bp	1.0	85.5
ndhF_p4	CAATTCCCGTTCTCAACCCA	AGAGCCGGTGAATCAGAAAC	50.0	50.0	58.1	57.9	2683-2851 bp	1.0	84.8
ndhF_p5	GTAACCCCCGGAACTTCTTC	AGAGCCGGTGAATCAGAAAC	55.0	50.0	57.9	57.9	2634-2803 bp	1.0	84.7